Usually means: Extra-large chromosomes with replicated DNA.
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We found 12 dictionaries that define the word polytene:

General (7 matching dictionaries)
  1. polytene: Merriam-Webster
  2. polytene: Oxford Learner's Dictionaries
  3. polytene: American Heritage Dictionary of the English Language
  4. polytene: Collins English Dictionary
  5. polytene: Wiktionary
  6. polytene: Dictionary.com
  7. polytene: TheFreeDictionary.com

Computing (1 matching dictionary)
  1. polytene: Encyclopedia

Medicine (4 matching dictionaries)
  1. polytene: Merriam-Webster Medical Dictionary
  2. online medical dictionary (No longer online)
  3. Hypermedia Glossary Of Genetic Terms (No longer online)
  4. polytene: Medical dictionary

(Note: See polyteny as well.)

Definitions from Wiktionary (polytene)

adjective:  (molecular biology) Having very large chromosomes with many chromatids (arms).
noun:  (molecular biology) A very large chromosome with many chromatids (arms); a chromosome exhibiting polyteny

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